How to Artificially Inseminate Gilts and Sows Post Cervically - YouTube

Hi, welcome to our Take 5 lesson on best PCAI practices.

A big thanks to our Take 5 sponsor Neogen in this demonstration we will be using Neogen's PCAI catheters

for insemination. Today we will be working with Dr. Kara Stewart an expert

and reproductive management of farm animals at Purdue University. She is a

PCAI expert. Well, hello Dr. Stewart how are you?

Hi, Dr. Sarah, I'm doing well glad

I can help out today.

Great Dr. Stewart will help walk us through the process

and give you tips for using Neogen's PCAI catheters. Are you ready to get

started?

I'm ready when you are!

Let's start at the beginning Dr. Stewart

what does PCAI anyway?

So PCAI or Post Cervical Artificial Insemination is a

process where we breed sows by depositing semen into the uterus which

is closer to the area where the eggs are located compared to conventional AI.

We use a special catheter that has an outer rod and that is similar to a

conventional AI rod in that it has a smaller diameter inner catheter that

actually passes through the cervix and into the uterus of the sow.

An advantage is that PCAI can be used to facilitate lower doses of semen per

insemination and faster insemination times this allows the best force to

inseminate more females which saves money per insemination and has the

possibility of saving labor time.

That's right Dr. Sarah

so during PCAI the boar is not required during the insemination process. He's

only going to be needed to heat check your sows and then during the

insemination you can gently squeeze the semen through the bag through the

catheter to speed up the process.

This difference is important for us to

understand isn't it? One of the biggest differences physiologically is that

during traditional AI you want the cervix of the south contracted around

the catheter and for the South's uterus to

all the semen through the catheter into her cervix. For this to occur the sow

needs stimulation from the bore as well as continued stimulation from the AI technician

That's right, Dr. Sarah, so the boar will only be needed to do

the heat checking when we're actually doing the insemination we will want the

cervix to be very relaxed which will occur about 15 minutes or more after the

boar has finished heat checking so the ideal time to do PCAI is between 15 and

40 minutes after heat checking.

Great! Well let's go through the steps.

Step 1 Prepare equipment.

We're going to be using Neogen's catheters today which

are color labeled so you know which type that you're going to pick up so the pink

Neogen catheters are used for sows and the green engine catheters are used

for gilts so you'll need to determine which animals you're on your way in to

breed you're also going to need to get some clean dry paper towels as well as

some non spermicidal gloves if your farm uses lubricant you will need to get that

prepared as well. Here on Purdue's farm we do not use lubricant but if you do

just apply that according to your standard operating procedures.

Of note is that when performing PCAI in the gilts the protocol is the same as in sows

with a few exceptions. First since the cervix and the uterus of the gilt is

much smaller than that of a post wean sow passing of the inner AI rod can be

challenging companies are producing catheters specifically designed for

gilts that accommodate their smaller reproductive tract. If you are breeding

gilts be sure to gather the right rods at this phase.

Step 2 Heat check

A sow that's in standing heat is going to lockup and basically be very rigid and

her front and back legs to bear the weight of the person heat checking. Her

ears will also tend to perk up and her vulva may be reddened and swollen with a

little bit of mucus in it.

Then we wait about 15 to 20 minutes to allow that

cervix to relax before we attempt to inseminate.

So while you wait this is when you should go gather your semen from the

semen cooler so you want to be cautious to only bring in the number of semen

doses that you need to inseminate. It's also a really good time to double-check

the temperature on those semen coolers.

Step 3 Clean the sow or gilts vulva

So right before you go in to inseminate you want to clean the sow or guilts vulva

make sure you remove any manure and just use a clean dry paper towel.

Step 4 Prepare the Neogen PCAI catheter for insertion

To prepare the Neogen catheter for insemination gently open the package and remove the catheter at

the end you want to be sure that you keep the catheter clean and don't let it

come in contact with any dirty floors or dirty bars around the sow. If your farm

uses lubricant now would be the time to apply it to the outer AI rod.

Step 5 insert the Neogen PCAI catheter into the sow.

So when you're ready to

insert the Neogene catheter you're gonna want to have non spermicidal gloves we

have Neogen's gloves on today use your fingers to gently spread open the vulva

insert the catheter at a 45 degree angle and then straighten it out to head into

the cervix you'll gently turn the catheter as you enter the cervix and

pull back gently to make sure you're locked in. Now you will slowly push the

inner catheter in half inch intervals you should feel the catheter move along

the rigid cervical folds until you insert the intrauterine catheter

completely into the sow if you encounter trouble when passing the inner rod in a

gilt you can simply remove the inner rod and breed conventionally using the outer rod.

Step 6 Attach the semen bag or tube to the

catheter and deposit the semen

So using temperature controlled semen you can go

ahead and attach either the tube or the bag to the end of the outer AI

catheter and gently squeeze to deposit the semen into the sow's uterus.

So if you see any backflow or any of that semen coming back out the sow's vulva

on to the floor then you need to stop what you're doing

and begin to manually stimulate the sow like you would during conventional AI

and let her accept that semen into her uterus.

If you are using a multi-dose

semen bag attach the dosage gun tip to the catheter and squeeze one dose slowly

into the catheter.

When you're inseminating the sow you want to hold

the bag or the dose up at a higher angle in order to try to prevent backflow from

happening and get the semen to deposit into her uterus. So you want to ensure

that you get the entire dose of semen into the sow. Sometimes you'll have to

actually remove the bag or the tube and put a little air in it in order to be

sure you get the entire dose of semen through the catheter. You'll know you've

got it all in there when you see the bubbles of air starting to pass through the catheter.

Step 7 Remove the Neogen PCAI catheter

So once you're done depositing semen and ready to remove the catheter you just put the end of the

catheter down towards the ground gently pull the whole entire unit out of the

sow and inspect the catheter for any leakage between the two AI rods or the

tip of the catheter for any signs of blood.

Step 8 Record completion according to your farms protocol at this farm once

you remove the catheter we will mark this sow and move down the line to the

next for insemination. Be sure to mark the sows record as well according to

your farms protocol. A key production tip is that you can inseminate up to five

sows at the same time as you do not need to stimulate the sows with flink

touching or back pressure this saves you time during insemination and helps

increase productivity on farm.

Step 9 Train employees on proper PCAI technique to get the best results.

As the procedures for doing PCAI are very different from conventional it's always important to train your employees

to ensure the greatest success. Though it typically takes about two days of

training to get employees competent at performing PCAI and you want to make

sure that they can pass the inner catheter through the cervix in order to

deposit that semen in the uterus accurately. So I hope this helps you

better understand PCAI and how it can improve productivity on your farm.

Well I think that covers it. Thank you so much for your time and expertise Dr. Stewart.

No problem Dr. Sarah see you later!

And thank you to our PCAI sponsor Neogen.

Technique matters when it comes to conception rates and total born

we appreciate your commitment to training on farm staff. Until next time

be safe and do good work!